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Isolation of Helicobacter pylori from Gastric Biopsy of Dyspeptic Patients in Ghana and In Vitro Preliminary Assessment of the Effect of Dissotis rotundifolia Extract on Its Growth

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dc.contributor.author Adinortey, Michael Buenor
dc.contributor.author Ansah, Charles
dc.contributor.author Adinortey, Cynthia Ayefoumi
dc.contributor.author Bockarie, Ansumana Sandy
dc.contributor.author Morna, Martin Tangnaa
dc.contributor.author Amewowor, Damian H.
dc.date.accessioned 2023-10-26T19:13:50Z
dc.date.available 2023-10-26T19:13:50Z
dc.date.issued 2018
dc.identifier.uri http://hdl.handle.net/123456789/10094
dc.description.abstract Helicobacter pylori (H. pylori) is a gram-negative bacterium that colonizes the human stomach. Infection with this microaerophilic bacterium causes gastric and duodenal ulcer. This study sought to isolate H. pylori, from gastric biopsy samples of dyspeptic patients in Ghana using a 2,3,5-triphenyltetrazolium chloride (TTC) dye incorporated medium method. This TTC dye method was further used in an antimicrobial susceptibility assay involving Dissotis rotundifolia extract (DRE). H. pylori were successfully isolated from gastric biopsy of dyspeptic patients. Pure cultures of H. pylori in 2,3,5-triphenyltetrazolium chloride (TTC) dye incorporated medium were seen as sparkling colonies. Isolates, identified as H. pylori, were gram-negative and urease, catalase, and oxidase positive and showed characteristic morphology as spiral-shaped bacteria under the microscope. The organisms were found to be susceptible to cephalothin and resistant to nalidixic acid. Above all, the observation that H. pylori grew only at 37∘C and not 25∘C or 42∘C affirms that the bacterium is neither Helicobacter cinaedi nor Helicobacter fenneliae. The anti-H. pylori study depicts a statistically lower zone of inhibition for DRE compared to standard drugs [amoxicillin and clarithromycin] (p<0.05), whereas metronidazole showed no zone of inhibition. This study reports the first successful isolation and culturing of H. pylori in Ghana using TTC dye. It also shows that DRE possess an in vitro anti-H. pylori activity and that DRE has some therapeutic potential against H. pylori infection en_US
dc.language.iso en en_US
dc.publisher Journal of Tropical Medicine en_US
dc.subject Helicobacter pylori en_US
dc.subject Gastric Biopsy en_US
dc.subject Dyspeptic Patients en_US
dc.subject Ghana en_US
dc.title Isolation of Helicobacter pylori from Gastric Biopsy of Dyspeptic Patients in Ghana and In Vitro Preliminary Assessment of the Effect of Dissotis rotundifolia Extract on Its Growth en_US
dc.type Article en_US


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