HPV with genital pathogens and antibiotics resistance genes among female sex worker

Abstract

Context: Genital infections and Sexually Transmitted Infections (STIs) remain a real public health problem in the world predominantly in sub-Saharan Africa. The purpose of this study was to determine co-infection of HPV, Neisseria gonorrhoeae (NG); Chlamydia trachomatis (CT); Mycoplasma genitalium (MG) and Trichomonas vaginalis (TV) among female sex workers in West Africa and to search antibiotics resistance genes. This study could serve as a support for the management of patients infected. Methods: The study took place in Ouagadougou in July 2019 and from June to July 2020. It was a cross-sectional study with descriptive and analytical aims. A total of 182 samples from sex workers of West and Central African origins, were analyzed by real-time PCR and resistance genes by classical PCR after DNA extraction. Data were entered and analyzed using the IBM SPSS software in its 21 version and Epi Info 6. Tables and figures were produced using IBM SPSS Statistics version 20 and Microsoft Excel 2007. Chi-square and fischer tests were used for comparisons Epi info version 7. with a significant difference for p 0.05. Results: These women, who came from nine different countries, were aged 17–50 years with an average age of 27.12 ± 6.09 years and had an average of 415.9 ± 75.2 sexual partners per year. HPV and vaginosis co-infection (NG, CT, MG and TV) was 85%. The prevalence of bacteria was: NG 13.74%, CT 11.54% and MG 11.54%. Among the HPV co-infections the most common were HPV/NG (15%), HPV/MG (12%), and HPV/CT (8%). %). The antibiotic resistance genes identified are: bla QNR B 24%, bla GES 22%, bla SHV 17%, bla CTX-M 13%; bla QNR S 1%. Conclusions: This study showed that the majority of sex workers of West and Central African origin working in Ouagadougou were infected with multiple STIs. This confirms that the presence of genital infections and STIs remains a real public health problem. The scale of these infections and the detection of associated resistance genes require increased surveillance of the molecular epidemiology of these pathogens.