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Natural antibody responses to Plasmodium falciparum MSP3 and GLURP(R0) antigens are associated with low parasite densities in malaria patients living in the Central Region of Ghana

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dc.contributor.author Amoah, L. E.
dc.contributor.author Nuvor, S. V.
dc.contributor.author Obboh, E. K.
dc.contributor.author Acquah, F. K.
dc.contributor.author Asare, K.
dc.contributor.author Singh, S. K.
dc.contributor.author Boampong, J. N.
dc.contributor.author Theisen, M.
dc.contributor.author Williamson, K. C.
dc.date.accessioned 2023-10-18T15:25:31Z
dc.date.available 2023-10-18T15:25:31Z
dc.date.issued 2017
dc.identifier.uri http://hdl.handle.net/123456789/9667
dc.description.abstract Background: Plasmodium falciparum genetic diversity and multiplicity of infection (MOI) are parasite features that have been suggested to influence the acquisition of protective immunity against malaria. This study sought to assess the relationship between MOI and parasite density (PD) in malaria patients living in the Central Region of Ghana and to determine whether naturally occurring antibody levels against P. falciparum GLURP (PF3D7_1035300) and MSP3 (PF3D7_1035400) antigens are associated with decreased parasite load. Methods: Dried filter paper blood blots were obtained from children and adults diagnosed with uncomplicated P. falciparum malaria. Microscopy was used to estimate P. falciparum parasite density and polymerase chain reaction (PCR) amplification of the polymorphic regions of msp1 (PF3D7_0930300) and msp2 (PF3D7_0206800) was used for parasite genotyping and MOI determination. ELISA was used to measure the serum IgG concentration of R0 fragment of GLURP (GLURP(R0)) and MSP3 antibodies. Results: All 115 samples were positive for P. falciparum by PCR using either the msp1 or msp2 genotyping primer sets. The most prevalent msp1 and msp2 alleles were KI and 3D7, respectively. The geometric mean (GM) for MOI determined by both msp1 and msp2 genotyping was 1.3 for the entire population and was generally higher in children than in adults. Seropositivity was estimated at 67 and 63% for GLURP(R0) and MSP3 antibodies, respectively, and antibody titers were negatively correlated with parasite density. Conclusions: The negative correlation between naturally occurring GLURP(R0) and MSP3 antibody levels and parasite density observed in this study suggest that augmenting the antibody response with the GMZ2 vaccine could enhance protection in the Central Region of Ghana en_US
dc.language.iso en en_US
dc.publisher Parasites & Vectors en_US
dc.subject Genetic diversity en_US
dc.subject Multiplicity of infection en_US
dc.subject msp1 en_US
dc.subject msp2 en_US
dc.subject MSP3 en_US
dc.subject GLURP en_US
dc.title Natural antibody responses to Plasmodium falciparum MSP3 and GLURP(R0) antigens are associated with low parasite densities in malaria patients living in the Central Region of Ghana en_US
dc.type Article en_US


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