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. Evaluating 18s-rRNA LAMP and selective whole genome amplifcation (sWGA) assay in detecting asymptomatic Plasmodium falciparum infections in blood donors

Show simple item record Aninagyei, Enoch Smith‑Graham, Stella Boye, Alex Egyir‑Yawson, Alexander Acheampong, Desmond Omane 2021-06-24T16:21:28Z 2021-06-24T16:21:28Z 2019
dc.identifier.issn 23105496
dc.description 11p:, ill. en_US
dc.description.abstract Background: Undesirable consequences of donor Plasmodium falciparum parasitaemia on stored donor blood have been reported. Therefore, it is imperative that all prospective blood donors are screened for P. falciparum infections using sensitive techniques. In this study, the sensitivities of microscopy, rapid diagnostic test (RDT), loop-mediated isothermal amplifcation (LAMP) assay and selective whole genome amplifcation (sWGA) technique in detecting P. falciparum infections in blood donors was assessed. Methods: Randomly selected blood donors from 5 districts in Greater Accra Region of Ghana were screened for asymptomatic P. falciparum infections. Each donor sample was screened with SD Bioline RDT kit for P. falciparum his‑tidine rich protein 2 and Plasmodium lactate dehydrogenase antigens, sWGA and 18s-rRNA LAMP. Crude DNA LAMP (crDNA-LAMP) was compared to purifed DNA LAMP (pDNA-LAMP). Results:A total of 771 blood donors were screened. The respective overall prevalence of P. falciparum in Ghana by microscopy, RDT, crDNA-LAMP, pDNA-LAMP and sWGA was 7.4%, 11.8%, 16.9%, 17.5% and 18.0%. Using sWGA as the reference test, the sensitivities of microscopy, RDT, crDNA-LAMP and pDNA-LAMP were 41.0% (95% CI 32.7–49.7), 65.5% (95% CI 56.9–73.3), 82.6% (95% CI 75.8–88.3) and 95.7% (95% CI 90.1–98.4), respectively. There was near perfect agreement between LAMP and sWGA (sWGA vs. crDNA-LAMP, κ=0.87; sWGA vs. pDNA-LAMP, κ=0.96), while crDNA LAMP and pDNA-LAMP agreed perfectly (κ=0.91). Goodness of ft test indicated non-significant difference between the performance of LAMP and sWGA (crDNA-LAMP vs. sWGA: x2=0.71, p=0.399 and pDNA-LAMP vs. sWGA: x2=0.14, p=0.707). Finally, compared to sWGA, the performance of LAMP did not difer in detecting sub-microscopic parasi‑taemia (sWGA vs. crDNA-LAMP: x2=1.12, p=0.290 and sWGA vs. pDNA-LAMP: x2=0.22, p=0.638). Conclusions: LAMP assay agreed near perfectly with sWGA with non-significant differences in their ability to detect asymptomatic P. falciparum parasitaemia in blood donors. Therefore, it is recommended that LAMP based assays are employed to detect P. falciparum infections in blood donors due to its high sensitivity, simplicity, cost-effectiveness and user-friendliness en_US
dc.language.iso en en_US
dc.publisher University of Cape Coast en_US
dc.subject 18s-rRNA-LAMP en_US
dc.subject Crude DNA LAMP en_US
dc.subject Purifed DNA LAMP en_US
dc.subject Selective whole genome amplification en_US
dc.subject P.falciparum histidine rich protein en_US
dc.subject Diagnostic indices en_US
dc.subject Crude DNA extraction en_US
dc.title . Evaluating 18s-rRNA LAMP and selective whole genome amplifcation (sWGA) assay in detecting asymptomatic Plasmodium falciparum infections in blood donors en_US
dc.type Article en_US

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