Abstract:
Heliotropium indicum is used traditionally as a remedy for conjunctivitis in Ghana.This study therefore evaluated the antiallergic
potential of an aqueous whole plant extract of Heliotropium indicum (HIE) in ovalbumin-induced allergic conjunctivitis and
attempted to predict itsmode of action.Clinical scores for allergic conjunctivitis induced by intraperitoneal ovalbumin sensitization
(100 : 10 𝜇g OVA/Al(OH)3 in phosphate-buffered saline [PBS]) and topical conjunctival challenge (1.5mg OVA in 10 𝜇L PBS) in
Dunkin-Hartley guinea pigs were estimated after a week’s daily treatment with 30–300mg kg−1 HIE, 30mg kg−1 prednisolone,
10 mg kg−1 chlorpheniramine, or 10mLkg−1 PBS. Ovalbumin-specific IgG and IgE and total IgE in serum were estimated using
Enzyme-Linked Immunosorbent Assay. Histopathological assessment of the exenterated conjunctivae was also performed.The 30
and 300 mg kg−1 HIE treatment resulted in a significantly (𝑝 ≤ 0.001) low clinical score of allergic conjunctivitis. Ovalbuminspecific
IgG and IgE as well as total serum IgE also decreased significantly (𝑝 ≤ 0.01–0.001). The conjunctival tissue in HIE
treated guinea pigs had mild mononuclear infiltration compared to the PBS-treated ones, which had intense conjunctival tissue
inflammatory infiltration. HIE exhibited antiallergic effect possibly by immunomodulation or immunosuppression.
