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Erythrocyte Shape Change Prevents Plasmodium falciparum Invasion

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dc.contributor.author Boampong, Johnson Nyarko
dc.contributor.author Manno, Sumie
dc.contributor.author Koshino, Ichiro
dc.contributor.author Takakuwa, Yuichi
dc.date.accessioned 2022-07-07T09:44:27Z
dc.date.available 2022-07-07T09:44:27Z
dc.date.issued 2007
dc.identifier.issn 23105496
dc.identifier.uri http://hdl.handle.net/123456789/8422
dc.description 8p:, ill. en_US
dc.description.abstract Normal erythrocytes have biconcave discoid shape that presents large surface area with higher cell surface to volume ratio than that of spherical shape. This appears to allow membrane internalization required for Plasmodium falciparum (Pf) invasion into erythrocytes. Indeed abnormal erythrocyte shape with decreased surface area to volume ratio such as hereditary spherocytosis limits invasion of the parasite. In the present study, using several agents to induce erythrocyte shape changes, we examined whether echinocytic shape change with membrane projections in opposite direction to membrane internalizations and/or stomatocytic shape change with decreased surface area to volume ratio that would be required for internalization, prevent Pf invasion. Having microscopically confirmed echinocytic and/or stomatocytic shape changes and also measured extensibility using an ektacytometer of the treated cells, subsequent Pf invasion assay was performed and parasitaemia determined. Both sodium flouride (NaF) and phospholipase A2 (PLA2) induced echinocytic change whereas phospholipase D (PLD), sphingomyelinase (SMase) and chlorpromazine (CPZ) caused stomatocytic change with decreased extensibility of erythrocytes. In both situations, Pf invasion was prevented, indicating that biconcave discoid shape of normal erythrocytes with high surface to volume ratio is required for membrane internalization when Pf invades into erythrocytes. en_US
dc.language.iso en en_US
dc.publisher University of Cape Coast en_US
dc.subject Plasmodium falciparum en_US
dc.subject erythrocyte en_US
dc.subject cell surface to volume ratio en_US
dc.subject cell shape change en_US
dc.subject membrane extensibility en_US
dc.title Erythrocyte Shape Change Prevents Plasmodium falciparum Invasion en_US
dc.type Article en_US


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