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Inhibition of Angiogenesis by Recombinant VEGF Receptor Fragments

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dc.contributor.author Ahmadvand, Davoud
dc.contributor.author Rahbarizadeh, Fatemeh
dc.contributor.author Iri-Sofla, Farnoush Jafari
dc.contributor.author Namazi, Gholamreza
dc.contributor.author Khaleghi, Sepideh
dc.contributor.author Geramizadeh, Bita
dc.contributor.author Pasalar, Parvin
dc.contributor.author Karimi, Hosein
dc.contributor.author Bakhtiari, Seyed Hamid Aghaee
dc.date.accessioned 2022-07-13T14:54:15Z
dc.date.available 2022-07-13T14:54:15Z
dc.date.issued 2010
dc.identifier.issn 23105496
dc.identifier.uri http://hdl.handle.net/123456789/8452
dc.description 7p:, ill. en_US
dc.description.abstract Background: Blocking vascular endothelial growth factor receptor (VEGFR) is a successful approach for inhibiting vascular endothelial growth factor (VEGF) signaling. Small molecules impairing the interaction of VEGF with VEGF receptors have been synthesized and evaluated in this research. Methods: In this study, we amplified and cloned the cDNA of VEGFR fragments. After expression of the fragments in Escherichia coli (E. coli), they were purified by immobilized metal affinity chromatography (IMAC). The biological activity of recombinant KDR fragments was evaluated by human umbilical vein endothelial cells (HUVEC) proliferation assay. Results: The sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PSGE) and immune-blotting results confirmed the production and purification of recombinant VEGFR fragments. The purified VEGFR2-III domain, VEGFR2-II-III domains, and VEGFR1-II domain showed 24%, 36%, and 27% inhibition effect in HUVEC proliferation assay, respectively. The recombinant VEGFR2-IIIII domains strongly inhibited formation of capillary-like structures (CLS). Conclusions: The produced recombinant proteins will serve as small soluble molecules with an inhibitory effect against VEGF in antiangiogenesis researches. en_US
dc.language.iso en en_US
dc.publisher University of Cape Coast en_US
dc.subject angiogenesis en_US
dc.subject VEGFR fragments en_US
dc.subject expression en_US
dc.title Inhibition of Angiogenesis by Recombinant VEGF Receptor Fragments en_US
dc.type Article en_US


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