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Geographical Distribution of β-Lactam Resistance among Klebsiella spp. from Selected Health Facilities in Ghana

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dc.contributor.author Quansah, Elvis
dc.contributor.author Barnie, Prince Amoah
dc.contributor.author Acheampong, Desmond Omane
dc.contributor.author Obiri-Yeboah, Dorcas
dc.contributor.author Mills, Richael Odarkor
dc.contributor.author Asmah, Ebenezer
dc.contributor.author Cudjoe, Obed
dc.contributor.author Dadzie, Isaac
dc.date.accessioned 2023-10-19T10:33:54Z
dc.date.available 2023-10-19T10:33:54Z
dc.date.issued 2019
dc.identifier.uri http://hdl.handle.net/123456789/9695
dc.description.abstract β-Lactam-resistant Klebsiella isolates continue to cause multidrug resistance infections worldwide. This study aimed to describe the geographical distribution of extended spectrum β-lactamase (ESBL), AmpC β-lactamase (AmpC), and carbapenemase production among 139 Klebsiella isolates recovered from patients at major referral health facilities in Ghana. The phenotypic methods of combined disc diffusion test, modified three-dimensional test, modified Hodge test (MHT), and combined disc test were performed for each isolate to detect ESBL, AmpC, carbapenemase, and metallo-β-lactamase (MBL) producers, respectively. Except for MBL, all other β-lactam resistance mechanisms were highest in the healthcare facilities situated in the northern belt of Ghana. Significant regional difference of ESBL producers was observed between the northern and middle belts as well as the northern and southern belts. Genotypic detection with polymerase chain reaction (PCR) revealed the presence of bla TEM 36/139 (25.9%), bla SHV 40/139 (28.8%), bla CTX-M 37/139 (26.6%), bla OXA-48 3/139 (2.16%), and bla NDM 1/139 (0.72%) genotypes. In conclusion, there were variations in β-lactam resistance among Klebsiella spp. from health facilities situated in the northern, middle, and southern belts of Ghana. The study provides preliminary evidence that emphasizes the need to direct more attention to antimicrobial resistance control, especially in the northern belt of Ghana. Findings from this study may be critical for creating and fine-tuning effective antimicrobial resistance control strategies and for informing accurate antibiotic prescription by practitioners. en_US
dc.language.iso en en_US
dc.publisher Tropical Medicine and Infectious Disease en_US
dc.subject β-lactamases; en_US
dc.subject Klebsiella spp.; en_US
dc.subject extended spectrum beta-lactamase; en_US
dc.subject AmpC; en_US
dc.subject carbapenemase; en_US
dc.subject metallo-β-lactamase en_US
dc.title Geographical Distribution of β-Lactam Resistance among Klebsiella spp. from Selected Health Facilities in Ghana en_US
dc.type Article en_US


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