University of Cape Coast Institutional Repository

Antiallergic effect of an aqueous leaf extract of Pistia stratiotes in murine model of ovalbumin‑induced allergic conjunctivitis

Show simple item record

dc.contributor.author Abokyi, Samuel
dc.contributor.author Koffuor, George Asumeng
dc.contributor.author Kyei, Samuel
dc.contributor.author Asiamah, Emmanuel A.
dc.contributor.author Atobiga, Clement Nsobire
dc.contributor.author Awuah, Agnes
dc.date.accessioned 2023-10-24T18:24:45Z
dc.date.available 2023-10-24T18:24:45Z
dc.date.issued 2014
dc.identifier.uri http://hdl.handle.net/123456789/9984
dc.description.abstract Aim: The aim was to investigate the antiallergic effect of an aqueous leaf extract of Pistia stratiotes (ALPS) in a murine model of ovalbumin (OVA)‑induced allergic conjunctivitis (AC). Materials and Methods: Prior to topical challenge (instillation of 1.5 mg OVA in 10 μL phosphate buffered saline into their conjunctival sacs) to induce AC, groups of sensitized Imprinting Control Region mice (injected IP, on day 1 and 7, with 0.2 ml solution of 100 μg OVA and 0.01 mg aluminum hydroxide in phosphate buffered saline), were treated with 5 mg/kg cetirizine, 10, 50 or 100 mg/kg of ALPS, or 2 ml/kg normal saline per os. Conjunctival redness, lid edema, tearing and lid scratching (clinical symptoms of AC) were scored. Serum OVA specific immunoglobulins were determined using ELISA. Histopathological assessment of the conjunctival mucosal tissue was conducted. The extract was screened for secondary plant metabolites. Results: Pretreatment with the extract significantly (P ≤ 0.05–0.01) and dose‑dependently reduced the scores for clinical symptoms, which were marked in vehicle‑pretreated mice. Pretreatment also lowered (P ≤ 0.01–0.001) serum OVA specific immunoglobulins. Mast cell infiltration and degranulation in conjunctival stroma (measured by an inflammatory score) in histopathological studies was also significantly low (P ≤ 0.05–0.01) on pretreatment. Conclusion: The ALPS exhibited interesting antiallergic activity and hence could be useful in managing AC. en_US
dc.language.iso en en_US
dc.publisher Pharmacognosy Research en_US
dc.subject Conjunctival redness, en_US
dc.subject lid edema, en_US
dc.subject mast cell degranulation, en_US
dc.subject mast cell infiltration, en_US
dc.subject tearing en_US
dc.title Antiallergic effect of an aqueous leaf extract of Pistia stratiotes in murine model of ovalbumin‑induced allergic conjunctivitis en_US
dc.type Article en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record

Search UCC IR


Advanced Search

Browse

My Account