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Chemical profile and in vivo toxicity evaluation of unripe Citrus aurantifolia essential oil

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dc.contributor.author Adokoh, Christian K.
dc.contributor.author Asante, Du-Bois
dc.contributor.author Acheampong, Desmond O.
dc.contributor.author Kotsuchibashic, Yohei
dc.contributor.author Armahb, Francis A.
dc.contributor.author Sirikyid, Ignatius H.
dc.contributor.author Kimurac, Keisuke
dc.contributor.author Gmakameb, Edward
dc.contributor.author Abdul-Raufb, Sey
dc.date.accessioned 2023-09-29T11:29:59Z
dc.date.available 2023-09-29T11:29:59Z
dc.date.issued 2019-06-22
dc.identifier.uri http://hdl.handle.net/123456789/8692
dc.description.abstract Citrus aurantifolia (Christm.) Swingle (syn. C. MEDICA var. ACIDA Brandis) (family: Rutaceae) essential oil is one of the cheapest oils found in local markets. Although, it is generally accepted as non-toxic to vital organs and cells, majority of people are cynical about it usage. Herein, the present study reports the chemical composition and in vivo oral toxicity study of unripe C. aurantifolia essential oil found in Ghana. The toxicity of C. aurantifolia essential oil extract was investigated via oral administration using two methods: The acute toxicity single dose study (SDS) and the repeated dose method. The oil exhibited no acute toxicity but in the sub-chronic studies, the effects was dose and time-dependent. Chemical profile investigation of the oil showed 9 constituent of phyto- chemicals (Germacrene isomers (61.2%), Pineen (14%), Linalool dimmer (2.9%), Bornane (11%), Citral (2.9%), Anethole (1.5%), Anisole (1.1%), Safrole (0.3%) and Demitol (0.6%)). Histopathological studies revealed con- ditions such as necrosis, edema and inflammatory reaction in the liver, spleen and kidneys. Marginal upsurge of biochemical parameters above normal and elevated levels of lymphocytes (35.20–46.40 g/dL) demonstrated mild toxicity among the 100 mg/kg and 500 mg/kg dose groups at the sub-chronic stage. Low levels of he- moglobin (13.60 to 12.70 g/dL), MCV (34.20–24.0 fL), MCH (40.20–36.40 g/dL) along with high levels of liver enzymes confirmed the mild toxicity of the oil at sub-chronic stage. These results demonstrate that, despite consideration of lime essential oil as safe, it can have mild hematotoxic, nephrotoxic and hepatotoxic effects. en_US
dc.language.iso en en_US
dc.publisher University of Cape Coast en_US
dc.subject Citrus aurantifolia en_US
dc.subject Biochemical analysis en_US
dc.subject In vivo toxicity en_US
dc.subject Histopathological en_US
dc.subject Hepatotoxicity en_US
dc.subject Nephrotoxicity en_US
dc.subject Hematological en_US
dc.title Chemical profile and in vivo toxicity evaluation of unripe Citrus aurantifolia essential oil en_US
dc.type Article en_US


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